Abstract: To investigate the possible application of carboxymethylatedlevan and its derivative on functional food, cosmetics and medicine, collagen peptides were used to modify carboxymethylated levan through the cross-linking reaction between carboxyl group and amino group.Moreover, the antioxidant activity and the moisture absorption and retention capacities of the modified product, as well as its effect on the viability of human skin keratinocyte cells ( Ha Ca T cells) were evaluated. After modified withcollagen peptides, the antioxidant activity of carboxymethylatedlevan was obviously improved. The scavenging rate of the product on ABTS⁺·was 6.11 times higher than that before modification, with the scavenging rate of 62.31% at 25 mg/m L.Though collagen peptides had some negative effect on the moisture absorption and retention capacities of the product, its modified carboxymethylatedlevan still had good moisture absorption and retention capacities.The moisture-absorbing rate of the product under the relative humidity of 43% and 81% was 11.96% and 21.54% at 72 h, respectively. The moisture retention capacity after desiccation in the silica gel chamber for 72 h was 60.48%. Furthermore, carboxymethylatedlevan modified with collagen peptides showed no cytotoxicity in the concentration of 0 ~ 1 mg/m L and could promote the proliferation of Ha Ca T cells.Under the stimulation of the modified product at 0.5 mg/m L, the cell viability of Ha Ca T cells reached 124.2%.The results showed that the modification with collagen peptides could improve the bioactivity of carboxymethylatedlevan, and extend its potential application on functional food, medicine and cosmetics.