Abstract: Objective: To observe the effects of fermented Chaenomeles speciosa Juice( FCJ) on the lipid metabolism and AMPK / SIRT1 pathway of non- alcoholic fatty liver disease( NAFLD) mice induced by high sugar and fat diet. Methods: Thirty- two SPF male Kunming mice were divided randomly into four groups: the control group,the model group,the high- dose FCJ- treated group( 10 m L / kg),and the low- dose FCJ- treated group( 5 m L/kg). After the treatment of three weeks,the blood and liver samples were collected.The activity of ALT in serum and triglyceride( TG) in liver tissues were detected with detection kits. The histology of liver tissues was observed with HE and red oil O staining. The mRNA and protein expression levels of p- AMPK and SIRT1 in liver tissues were measured with RT- PCR and Western Bloting.Results: Compared with the normal group,hepatic lipid accumulation of model group was severe showed by histopathological examination,the serum levels of ALT and TG in the liver tissue were increased significantly( p < 0.05),the expression levels of SIRT1,Fox O1 mRNA and SIRT1,P- AMPK protein of liver tissues were significantly decreased( p < 0.05). Compared with the model group,the hepatic lipid accumulation in FCJ- treated groups was obviously ameliorated,the serum ALT and TG levels of FCJ- treated groups were significantly decreased( p < 0.05),the expression levels of hepatic SIRT1,Fox O1 mRNA and p- AMPK,SIRT1 protein in FCJ- treated groups were significantly up- regulated( p < 0.05). The effect of high dose group was better than the low dose group,but the two were not statistically significant. Conclusion: FCJ canameliorate the disorder of lipid metabolism in the NAFLD mice induced by feeding high sugar and fat diet,and the mechanism may be related to the activation of AMPK / SIRT1 pathway in hepatocytes.