Abstract: To effectively improve the production of the Bohai sea- 9145 lipase,single factor experiment,PB experiment and BB surface response experiment were used to optimize the fermentation conditions of the recombinant strain Bohai sea-9145.The optimal conditions were as follows: the initial p H value of the medium was8.0,the inoculation amount was 1.5%,and after 3.5 hours of incubation,the expression of the lipase was induced by adding IPTG to a final concentration of 0.5 mmol / L at 16 ℃ for 15 hours with the shaking speed of 200 r / min.After optimization,the final activity of the recombinant lipase was increased by 9.72 times.