大鲵活性肽酶法制备工艺优化及抗氧化性分析

Optimal enzymatic preparation conditions and antioxidative activity of giant salamander bioactive peptide

摘要: 研究了碱性蛋白酶水解制备大鲵活性肽的工艺条件以及大鲵活性肽的理化性质。以水解度为指标,通过Box-Behnken实验设计方法,确定最佳工艺条件为:酶解温度50℃,酶解时间1.5 h,酶添加量70 U/g,p H8.91,液料比2∶1（m L/g）,蛋白酶解液的水解度值为DH=19.61%±0.12%。所得大鲵活性肽的蛋白质含量为52.40%±1.28%。HPLC分析可知该大鲵活性肽的分子量多分布在1000.00 u以内。在30 mg/m L,大鲵活性肽对羟自由基和超氧阴离子自由基的清除率分别为81.83%、56.27%。

Abstract: In this paper,enzymatic preparation conditions of giant salamander bioactive peptide and its physicochemical properties were studied. Based on the degree of hydrolysis,the preparation method was optimized by response surface methodology. The optimal temperature,the reaction time,the ratio of liquor to material,enzyme amounts,and p H were 50 ℃,1.5 h,2 ∶ 1( m L / g),70 U / g,and p H8.91,respectively,the highest degree of hydrolysis was obtained,up to 19.61% ± 0.12%. Protein content of the bioactive peptide was 52.40% ±1.28% and the molecule weight of the most bioactive peptide was below 1000 u. At the concentration of 30 mg / m L,the scavenging rates of giant salamander bioactive peptide against hydroxyl radical and superoxide anion were81.83% and 56.27%,respectively.