Abstract: The culture conditions of Pseudozyma sp.JCC207 from Antarctic and the optimization of the extractive technology for its trehalose were studied in this paper. Effects of culture temperature and initial p H of culture medium on Pseudozyma sp.JCC207 growth were explored by single factor tests,and the optimization of extraction condition of yeast trehalose by rchloroacetic acid( TCA) was determined using response surface method( RSM). The results showed that the optimum culture temperature was 25 ℃,and initial p H value of 5 to 9 impacted little on yeast growth,and the optimal extraction conditions were obtained as follows: TCA concentration 0.6 mol / L,TCA volume 15.20 m L,and extraction time 29 min. The extraction amount was 17.72 mg from one gram wet cells of Pseudozyma sp. JCC207.Function test indicated that 0.5% trehalose solution had a better conservation capacity for yeast in 5 days compared with 20% glycerol and 10% dimethyl sulphoxide( DMSO).