黄腐酸对雨生红球藻(Haematococcus pluvialis LUGU)虾青素积累的影响

Effects of fulvic acid on astaxanthin accumulation of Haematococcus pluvialis LUGU

摘要: 通过形态学以及18S r RNA的分析,筛选鉴定出了一株新的雨生红球藻Haematococcus pluvialis LUGU,并以其作为研究对象,研究了不同质量浓度的黄腐酸(FA)对胁迫培养条件下细胞生长和虾青素产量的影响。结果表明,5 mg/L和10 mg/L的FA不仅促进了藻细胞的生长(生物量浓度分别比对照组增加了4.26%和10.58%),而且能够显著提高虾青素的含量(最大含量分别达到20.82和12.23 mg/L)。其中,5 mg/L的FA诱导效果最为明显,虾青素产量(37.46 mg/g)比对照组(20.90 mg/g)提高79.23%。实验表明,黄腐酸在适当浓度下能够显著促进藻细胞内虾青素的积累,可作为一种有效的诱导子应用于虾青素的生产。

Abstract: A novel strain identified by the morphology and 18 S r RNA namely,Haematococcus pluvialis LUGU,was used to explore the effect of fulvic acid(FA) on the growth of algae and the production of astaxanthin in this study. As a result,the biomass of H. pluvialis increased by 4.26% and 10.58% than controls and the astaxanthin content reached 20.82 and 12.23 mg/L in 5 and 10 mg/L FA-treated culture,respectively. Particularly,5 mg/L FA enhanced 79.23% with 37.46 mg/g in the production of astaxanthin more than that of control group(20.90 mg/g). The result indicated that FA was a effective factor to enhance the production of astaxanthin at appropriate concentration.