Abstract: To study the inhibitory effects of the zinc oxide nanoparticles on T. roseum,Fusarium spp.,Alternaria,which caused pink rot,Fusarium rot,black spot of postharvest muskmelon,respectively. The solid phase and liquid phase zinc oxide nanoparticles at different concentration and T. roseum,F. Sulphureum,A. alternate were used in vitro and vivo study. In vitro,the results showed that the significant inhibitory effect on mycelial growth and spore germination was found at different states of zinc oxide nanoparticles. The inhibition effect was greatly enhanced with the increase of the zinc oxide nanoparticles' s concentration. The inhibitory effect of the solid phase zinc oxide nanoparticles was more effective than the liquid phase one,which showed the most effect for T. roseum,then F. sulphureum,A. alternate. In vivo,it indicated that the solid phase and liquid phase zinc oxide nanoparticles had the significant inhibitory effect on the diameter of melon disease spot,which was caused by T. roseum,F. sulphureum,A. alternate,respectively. So zinc oxide nanoparticles could directly inhibit the major of pathogens to decrease disease of postharvest muskmelon,besides the inhibitory effect of solid phase zinc oxide nanoparticles was better than the liquid.