Abstract: The response surface methodology experiments were used to optimize hyperoside,isoquercitrin,and quercetin from Abelmoschus manihot(L.) Medic. Based on single factor experiments,including ultrasonic power,extraction time,ethanol concentration,extraction temperature,solid-liquid rate,response surface method was used to establish polynomial regression equation model with 50 ℃ and 0.1∶15 g/m L. The optimal condition were340 W,extraction time 60 min,and ethanol concentration 60%. Under such extraction technological conditions,the extracting rates of hyperoside,isoquercitrin and quercetin were 4.97 mg/g(RE=2.5%),4.84 mg/g(RE=4.5%)and 2.02 mg/g(RE=6.9%). Used response analysis to obtain the optimum process conditions of extracting surface design could be used to provide theoretical reference for industrialized extraction.