普洱熟茶中黄酮醇类物质杨梅素、槲皮素和山奈酚的分离纯化
Separation and purification of flavonols myricetin, quercetin and kaempferol in cooked Pu- erh tea
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摘要: 本文以云南普洱熟茶为实验材料,以不同浓度乙醇水溶液为洗脱液,利用MCI GEL CHP 20P（75150μm）树脂色谱柱和SephadexTMLH-20葡聚糖凝胶色谱柱,并结合高效液相色谱（HPLC）法,对普洱熟茶中主要黄酮醇类物质进行了分离和定性。结果表明:普洱熟茶提取液浓缩后过MCI GEL CHP 20P（75150μm）树脂色谱柱,经不同浓度（10%,30%,50%,70%,90%）乙醇溶液洗脱分离后,将获得的70%和90%的洗脱液过SephadexTMLH-20葡聚糖凝胶色谱柱,70%的洗脱液经90%乙醇溶液洗脱分别获得了HPLC纯度为96.0%的杨梅素和HPLC纯度为98.5%的槲皮素;90%的洗脱液经70%的乙醇溶液洗脱获得了HPLC纯度为95.6%的山奈酚。Abstract: In this paper, Yunnan cooked Pu- erh tea was used as experiment material, separation and purification of flavonols were investigated with different concentrations of ethanol as eluent by column chromatography of resin and SephadexTMLH-20, combined with high- performance liquid phase chromatography ( HPLC) method, and the main flavonols were isolated and characterized. The results showed that: the extract of cooked Pu- erh tea with different concentrations ( 10%, 30%, 50%, 70%, 90%) of ethanol by MCI GEL CHP 20P ( 75 150 μm) , the myricetin with the HPLC purity of 96.0% and the quercetin with the HPLC purity of 98.5% were isolated from elution 70%eluent with 90% ethanol while kaempferol with the HPLC purity of 95.6% was isolated from elution 90% eluent with70% ethanol by SephadexTMLH-20.
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