Abstract: This method gave an account that extracted by alkaline solution,extracted by hexane to remove the fat,back-extraction with ethyl acetate-isopropanol under acid condition,purified by mixing anionic solid-phase extraction(SPE),finally,using liquid chromatography(HPLC) for testing.The three kinds of compound sodium nitrophenol components concentration and the peak area was linear correlation within the scope of 0. 05 ~1.0 μg/m L. The correlation was 0.999. Through the extraction,back extraction and a variety of means such as solid phase extraction purification,the sample almost had no interference,so the detection limit could reached0.003~0.005 mg/kg. Through the standard addition test of 0.25,0.5,1.0 μg,its recovery was between 81%~95%,relative standard deviation was less than 10%.