桑黄子实体多糖、黄酮和多酚含量与抗氧化活性相关性

Relationship between the content of polysaccharides, flavonoids and polyphenols from the sporocarp of Phellinus linteus and the antioxidant activity

摘要: 探讨子实体抗氧化的物质基础。通过提取和萃取得到7种组分:水提物(WE)、醇提物(AE)、粗多糖(CP)、醇沉上清干物(RL)和二氯甲烷(MCE)、乙酸乙酯(EAE)、正丁醇(n BE)萃取物,采用FRAP值法测定体外抗氧化能力,分光光度法测定多糖、黄酮和多酚含量。结果表明:抗氧化活性物存在于醇提物中,抗氧化活性和多酚、黄酮含量有关,并呈线性正相关(R2为0.9977和0.9950),多糖在抗氧化中所起作用小,酚类、黄酮类为其主要抗氧化活性物质。

Abstract: To evaluate the antioxidant activity of active compounds in phellinus linteus, ferric reducing ability of plasma (FRAP) was used to evaluate the antioxidant activity, spectrophotography was used to determine the content of polysaccharides, flavonoids and polyphenols in seven extracts (water extract, alcohol extract, crude polysaccharide, residual liquid after alcohol sink and methylene chloride, ethyl acetate, n- butanol extracts respectively) . The results showed that, the antioxidant components were found in alcohol-soluble part, the antioxidant activity appeared positive correlation with the content of polyphenols and flavonoids (R2=0.9977 and0.9950 respectively) , polysaccharides played a less significant role than that of polyphenols and flavonoids, polyphenols and flavonoids were the main antioxidant components.