Abstract: HPLC has been applied to determine the contents of ginsenoside Rb1,ginsenoside Re,salidroside,acanthopanax B and acanthopanax E in the mixture extracts of Panax quinquefolius,Rhodiola rosea,and Eleutherococcus senticosus.The samples extracted with 60% alcohol were separated on a Kromasil 100-5 C18column( 250 × 4.6mm,5μm),and were eluted with 0.1% phosphoric acid-acetonitrile at a flow rate of 0.9m L/min under gradient elution conditions. The results showed that all of the saponins in the mixture extracts were well separated on HPLC. The linear ranges of the ginsenoside Rb1 and ginsenoside Re,salidroside,acanthopanax B and acanthopanax E were 80 ~ 800,80 ~ 800,40 ~ 400,20 ~ 160μg/m L and 40 ~ 320μg/m L,respectively. Their average recovery rates were 99.5% ~ 106.8%,with the relative standard deviations of 0.5% ~ 2.3%. In conclusion,the proposed method had a good linearity and sensitivity which was suitable to detect simultaneously the contents of the ginsenoside Rb1,ginsenoside Re,salidroside,acanthopanax B and acanthopanax E in the mixture extracts or formulations.