Abstract: According to the inv A gene of Salmonella spp.,a pair of primers was designed to establish a real- time PCR.The rapid,specific,sensitive detection of Salmonella spp. was achieved,and the evaluation of detection sensitivity,specificity and repeatability was carried out. This method had good specificity. The limit of detection of the method was 86 copies,correlation coefficient of the standard curve was 0.999 and amplification efficiency was104%.Different concentrations of plasmid repetitive amplification coefficients of variation were less 3%,accord with standard preferred for moderately laboratory by WHO,indicating a good reliability. The SYBR Green I real- time PCR detection technology was rapid,simple,specific,and sensitive,which may be widely applied in the detection of Salmonella spp. in the field of food sanitation supervision,commodity inspection and detection,clinical diagnosis,etc.