响应面法优化超声辅助醇提黄芪黄酮最佳工艺

Optimization of ultrasonic assisted alcohol extraction process of flavonoids from Radix Astragali by response surface methodology

摘要: 采用超声辅助醇提法提取黄芪中的黄酮,利用响应面法对黄芪黄酮提取工艺进行优化。在单因素实验基础上,通过响应面法对影响黄芪黄酮提取得率的乙醇浓度、料液比、提取温度、超声时间四个因素进行优化,确定最佳工艺条件为:乙醇浓度60%、料液比1∶20(g∶m L)、提取温度76℃、超声时间30min,黄酮得率为3.30%。

Abstract: Response surface methodology was used to optimaize the ultrasonic assisted alcohol extraction of flavonoids from Radix Astragali. On basis of single factor experiment, optimize the ethanol concentration, solidliquid ratio, extraction temperature, and ultrasonic time which influence the yield of flavonoids. The optimum technological conditions were determined as follows :the ethanol concentration 60%, solid-liquid ratio 1 ∶20 (g∶m L) , extraction temperature 76℃, ultrasonic time 30 min, and the flavonoids yield was 3.30%.