Abstract: The shelf-life prediction model of vacuum packing tilapia were established at different temperatures (273, 277 and 283 K, respectively) during storage. The indicators of Total Viable Count (TVC) , Total-Volatile Basic Nitrogen (TVB-N) and 2-thiobarbituric acid (TBA) were measured at the different storage temperature.The kinetic model between vacuum packing tilapia's quality and storage time was established based on the Arrhenius equation. The Activation energies (EA) and the rate constants (k0) of TVC, TVB-N and TBA were53.5kJ/mol and 4.390×108, 25.9kJ/mol and 8.96×103, 29.3kJ/mol and 4.92×104, respectively. The results showed that the higher the temperature arrived, the faster reaction rate did, while the relative error between the shelf-life model prediction value and the actual value was within ±10%. According to the values of TVC, TVB-N and TBA from 273 K to 283 K, vacuum packing tilapia's shelf-life could be predicted accurately.