Abstract: Primers specific to swine was designed for species-specific COⅠgene-amplification from barcoding was proposed by CBOL. Simultaneously in order to prevent false negative results, GCG gene fragment was used for the internal positive control (IPC) . Gene products of swine and GCG were represented in two melting peaks generated simultaneously at temperatures of 79.2℃ and 75℃ respectively. The specificity of the method was evaluated using template DNAs from bovine, chevon, chicken, rabbit, rat and NTC, whereas only amplification products of GCG gene were obtained. DNA sequencing results showed that the sequence of swine-specific products was 245bp, and which were 100% matching the corresponding sequence in the GenBank. The LOD of this method was 0.001%.