Abstract: Under 10℃ condition, the influence of different nitrogen source, carbon sources and buffer on the growth of two low temperature lactic acid bacteria 3m- 1 ( Lactobacillus plantarum) and 8m- 9 ( Leuconostoc mesenteroides) in cabbage basal medium were studied, and the orthogonal experiment was used to optimize the multiplication factor, the optimization cabbage medium of 3m-1 and 8m-9 were obtained as follows: peptone 1%, beef extract 0.5%, lactose 1.0%, glucose 1.5%, K2HPO40.2% were added into cabbage basal medium; peptone1.5%, beef extract 1.0%, lactose 0.5%, glucose 1.5%, K2HPO40.2% were added into cabbage basal medium.After cultivation in optimization cabbage medium for 72h at 10℃, the viable counts of 3m- 1 and 8m- 9 were 6.05 ×109cfu /mL and 7.35 × 109cfu /mL, respectively, which was about 6.1 times and 10.4 times higher than that in cabbage basal medium, but was similar to that in MRS medium.The research results had guiding siginifacance in preparation of low lactic acid bacteria starter, and also laid a foundation for producing pickles under low temperature.