Abstract: In order to clear the aroma components and in vitro functional activities of Kuding tea, by GC-MS analysis, DPPH free radical and hydroxyl radical assays, antimutagenicity test, AGS and HT-29 cancer cells proliferation inhibitory test, there were fifteen kinds of components were found in aroma components of Kuding tea, they were 2-imino-3-methyl-6-nitrobenzothiazoline, heptane, 2, 4-dimethyl-, decane, 2-ethylacridine, ergoline-8-carboxamide, 9, 10-didehydro-6-methyl-, (8.beta.) -, benzoic acid, methyl ester, 1-dimethyl the xylsilyloxyocta decane, benzothiazole, 2- (5-chloromethyl-1, 3, 4-oxadiazol-2-yl) -6-methoxy-;dimethyl phthalate, glycidol;propanamide, 8-dimethyl amino-1, 3, 7-trimethyl-3, 7-dihydropurine-2, 6-dione, phthalic acid, butyl undecyl ester, hexadecanoic acid, methyl ester and 2-amino-1- (o-methoxyphenyl) propane. And Kuding tea showed antioxidant, antimutagenicity test and anticancer effects. This study supplied the theoretical foundation for popularization of Kuding tea.