Abstract: Objective:To study the effect of Prunus maackii fruit anthocyanins on Nrf2/Keap1 pathway. Methods:The HepG2 cells were treatet with concentration of 0.05, 0.2, 0.8mg/mL Prunus maackii fruit anthocyanins solution for 24, 48, 72h respectively. The growth inhibition rate of HepG2 cells and the content of glutathione (GSH) , activity of phosph oinositol-3-kinase (PI3K) of each group cells were determined by the method of MTT. The real-time fluorescent quantitative RT-PCR method was used to study the effect of Prunus maackii fruit anthocyanins on Nrf2, Keap1, GSTP1 in HepG2 cells in genetic expression. Results:The results showed that as the content of anthocyanins increased, the content of GSH of HepG2 cells deceased, and the activity of glutathione s-transferases (GSH-ST) and PI3K decreased significantly. The Nrf2, Keap1, GSTP1 gene expression level dropped. Conclusion:the Prunus maackii fruit anthocyanins could reduce antioxidant capacity of HepG2cells by adjusting the Nrf2/Keap1 pathway.