黑鱼酶解产物肽的制备工艺研究

Study on the preparation technology of the enzymatic peptides of snakeheads

摘要: 以黑鱼为原料,探讨了黑鱼蛋白酶解产物肽的制备工艺,通过单因素实验和正交实验得出了黑鱼蛋白酶解的最佳工艺条件:用碱性蛋白酶酶解黑鱼的最优组合为加酶量1.5%,温度50℃,底物浓度1.5%,起始pH为9.0,酶解后的多肽含量是29.5%。

Abstract: Snakeheads were used as raw materials to discuss the preparation technology on the enzymatic peptides.Its optimum process conditions were concluded by single factor and orthogonal tests. The optimal portfolio which used alkaline protease to make snakeheads enzymatic was 1.5% ( enzyme dosage) , 50℃ ( temperature) , 1.5% ( concentration of substrate) , 9.0 ( pH) .The content of polypeptide was 29.5% after enzymolysis.