Abstract: Yeast strains GD9 of Yacai which was obtained by screening and domestication used as objective strain in this study. Based on single factor experiment, the optimized fermentation condition for yeast strains GD9 of Yacai was studied by using Box-Behnken condition and response surface methodology. Result showed that the optimized fermentation condition for yeast strains GD9 of Yacai as follow:inoculation time 22h, fermentation temperature 26℃ and pH 3.7, and under this optimal condition, OD620 of the GD9 strains reached 2.05. Furthermore, the established response surface model could accurately predict the different response conditions on OD620 of the GD9 strains.