柴达木盆地不同生长期菊芋叶片绿原酸含量变化规律研究
Research of the contents variation of chlorogenic acid in the leaves of Helianthus tuberosus L.during different growth stages in Chaidamu
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摘要: 本研究以柴达木盆地不同生长期菊芋叶片为研究材料,建立了菊芋叶片绿原酸HPLC快速定量检测方法,并对柴达木盆地菊芋叶片绿原酸含量随生长期变化规律进行研究。在选择流动相时,本文对测定条件进行优化,获得了较好的分离度,并进行了标准曲线的绘制。对不同采集时间菊芋叶片绿原酸含量测定结果表明,不同生长期菊芋叶片绿原酸含量差异显著,最高月份含量可达到最低月份含量的2倍,8月1日至10月15日菊芋叶片绿原酸含量呈上升趋势,至10月15日达到最高(叶片干重的0.83%),随后逐渐降低。以上实验数据表明,柴达木地区作为绿原酸提取原料的菊芋叶片采收期以10月15日左右为宜。Abstract: In this study, the rapid HPLC quantitative detection method of chlorogenic acid, and the contents variation of chlorogenic acid in the leaves of Helianthus tuberosus L. during different growth stages in Chaidamu were studied.In the choice of the mobile phase, the determination conditions were optimized, and obtained a better resolution.In addition, the standard curveand was carried out.The results showed that the chlorogenic acid contents in the leaves of Helianthus tuberosus L.during different growth stages had significant differences.The chlorogenic acid contents in the leaves of Helianthus tuberosus L.during August 1st to October 15th had an upward trend, and the highest content was reached on October 15th.The above experimental data indicated that the harvest period of Helianthus tuberosus L.should be determined around the October 15th.
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