Abstract: Euryale petioles and pedicel collected from 9 regions of Jiangsu province were detected using an automatic amino acid analyzer. The amino acid compositions of 9 samples were analyzed and compared. The nutritional value was evaluated.The results showed that the average content of total amino acid was 5.19g /100g.All samples contained 17 kinds of amino acids and included 7 kinds of essential amino acids in a balanced ratio, which meeting the recommendation of the WHO /FAO. Amino acid contents of samples from different origin were different.Therefore, Euryale petioles and pedicel is an aquatic vegetable rich in amino acid.The development and utilization of it should be done for its higher nutritional value.