Abstract: Test methods of fatty acids in marinated meat by gas chromatography-quadrupole mass spectrometry.Extracted by accelerated solvent extractor, 1 % H2 SO4- methanol solution method methyl esterification, and determined by gas chromatography mass spectrometry.The sample added recoveries was 80.2% ⁹6.2%, RSD was 2.4%⁶.8%.32 kinds of fatty acid methyl ester a good linear relationship, correlation coefficient was 0.9915⁰.9990.Detection limit was 0.02⁰.14μg/kg, limit of quantitation was 0.06⁰.46μg/kg.The method which was rapid, accuracy and high sensitivity could be used to test several fatty acid pickled meat food.