Abstract: A new method for water extraction from wine was developed using freeze-centrifugation for stable hydrogen and oxygen isotope ratio determination of water in wine. The extracted water contained little organic material.According to the simulation results, stable hydrogen and oxygen isotope ratio of water in wine did not change after the extracting process.Therefore the water extracted from wine by the freeze-centrifugation method can be used for stable hydrogen and oxygen isotope ratio determination of water in wine.