同时蒸馏萃取-气质联用分析艾草挥发性成分

Analysis of volatile components of Artemisia argyi by simultaneous distillation extraction and gas chromatography-mass spectrometry

摘要: 采用同时蒸馏萃取法,并结合气相色谱-质谱联用进行提取并分析艾草中的挥发性成分。实验结果表明,以乙醚为萃取溶剂时,使用DB-WAX型号毛细管色谱柱,鉴定出62种物质,而通过毛细管色谱柱HP-5鉴定出89种物质;以二氯甲烷为萃取溶剂时,在DB-WAX中经GC-MS鉴定出35种物质,在HP-5中鉴定出106种物质。使用两种萃取剂及两种不同极性的色谱柱共鉴定出159种物质,其中含量较大的有桉油精、石竹烯氧化物、石竹烯、3,3,6-三甲基-1,5-庚二烯-4-酮、3,3,6-三甲基-1,5-庚二烯-4-醇等。

Abstract: Volatile compounds of Artemisia argyi were extracted and analyzed by Gas Chromatography-Mass Spectrometry (GC-MS) with the method of simultaneous distillation extraction (SDE) . When taken diethyl ether as extraction solvent, 62 compounds were identified by GC-MS in the DB-WAX column, and 89 compounds in the HP-5 column;when taken methylene chloride as extraction solvent, 35 compounds were identified by GC-MS in the DB-WAX column, and 106 compounds in the HP-5 column. Totally, 159 compounds were verified by SDE-GC-MS with the two extraction solvent in the two columns. The prominent compounds were Eucalyptol, Caryophyllene oxide, Caryophyllene, 3, 3, 6-trimethyl-1, 5-heptadien-4-one, 3, 3, 6-trimethyl-1, 5-heptadien-4-ol, etc.