Abstract: Under different concentration (water control, 0.5%, 1.0%, 2.0%) of calcium chloride, effects of calcium on storage physiologies of Agaricus bisporus during low temperature circumstances (0～2℃ reserve temperature, 90%～95% relative humidity) were studied. The results indicated that 0.5% of calcium chloride treatment could effectively inhibit the production rate of superoxide anion and lipoxygenase, improve the activities of peroxydase, superoxide dismutase and catalase, and reduce the production of polyphenol. While 1.0% CaCl 2 treatment could improve peroxydase activity and lower the activity of polyphenol, but had no positive effects on other physiologies. 2.0% CaCl 2 treatment had little effects on Agaricus bisporus and couldn’t delay senility of it. To sum up, the treatment of 0.5% CaCl 2 could be used in delaying senility of Agaricus bisporus, and possess of popularization and application value widely.