Abstract: To study the effect of collagen oligopeptid on cell proliferation and synthesis of hyaluronic acid in cultured human skin fibroblast cell, and the effect of collagen oligopeptid on type Ⅰ collagen secretion of mouse skin.Proliferation viability was detected by WST-8 assay.ELISA was used to detect the secretion of hyaluronic acid of cultured human skin fibroblast.Collagen oligopeptid were given to mice and D-galactosewere injected into mice for six weeks.Then, determine the type Ⅰ collagen secretion of mouse skin.The results of the experiment showed that the collagen oligopeptidcould stimulate the human skin fibroblast proliferation (p<0.01) , and increase the hyaluronic acid and typeⅠcollagen secretion (p<0.05) .Collagen oligopeptid from tilapia fish scale could stimulate human skin fibroblast proliferation, and meanwhile increase typeⅠ collagen and hyaluronic acid secretion.