二次旋转正交组合设计优化羊骨蛋白酶解工艺

Optimizing enzymatic hydrolysis conditions of sheep bone protein through quadratic orthogonal rotation combination design

摘要: 以新鲜羊骨为原料,选用碱性蛋白酶和风味蛋白酶按序分步水解羊骨蛋白,采用二次旋转正交组合设计优化双酶水解羊骨蛋白工艺条件。结果表明,在初始pH为8.5,初始温度为55℃,羊骨蛋白的最佳酶解方案为:底物浓度为10%,碱性蛋白酶添加量为3000u/g,作用时间为5h;风味蛋白酶添加量为3500u/g,作用时间为3.5h。

Abstract: The fresh sheep bone was hydrolyzed with alcalase and flavourzyme.The optimization of hydrolysis conditions of sheep bone protein was carried out and mathematical model was established through the quadratic regression orthogonal rotary combination design.The results showed that when the initial pH8.5 and temperature was 55℃, the optimal conditions were alkaline protease dosage of 3000u/g, the role of alkaline protease time was 5h, flavourzyme addition level was 3500u/g, the role of alkaline protease time was 3.5h, the substrate concentration was 10%.