Abstract: The fatty acids composition of crude fat which was extracted by Soxhlet extractor from 8 tomato fruit samples, were determinated by gas chromatography (GC) after modified to methyl esters, with 37 kinds fatty acid methyl ester mixture.The results showed that:the content of dry matter in tomato fruits was 5.86% on average, the content of seeds 0% ～ 0.51%, the content of crude fat 6.97% on average.There were significant differences on total fatty acids among tomato fruit samples, up to 10 times.The content of total fatty acids in tomato fruits was positively related with seed weight to a certain extent.The relative content of fatty acids in tomato fruits also had certain differences.The major fatty acid components in fruits identified were as follow:hexadecanoic acid 13.2% ～ 26.4%, stearic acid 0% ～ 6.2%, oleic acid 0% ～ 27.5%, linoleic acid 50.5% ～ 70.9%, linolenic acid 2.5% ～ 20.0%.The results will provide a considerable aid for tomato breeding, cultivation and comprehensive utilization.