Abstract: Taking the wild plum in Guizhou karst area as materials,the contents and compositions of amino acids were assayed using the automatic amimo acid analyzer. The results showed that the wild plum contained 23 kinds of amino acids,of which 6 were essential amino acids,2 kinds of children essential amino acids. The highest content of total amino acids in the fruit Prunus mume was the Libo-14,which was 10925.31 mg/100 g,which was significantly higher than that of the other plants and cultivars. The essential amino acids and the children essential amino acids were also abundant,and their respectively contents were 1226.24,957.33 mg/100 g. The most of the amino acid species in the fruit Prunus mume were Libo-11 and Libo-16,containing 21 kinds of amino acids. Arginine(arg)content was the highest among abundant medicinal amino acids in fruit Prunus mume. The content of branched chain amino acids in most fruits was also rich,and the Libo-10 was up to 212.05 mg/100 g. In addition,it was found that the content of flavor amino acids were higher in most fruits,and accounted for about 50% of the total amino acids. The contents of delicious amino acids(866.63 mg/100 g)and sweet amino acids(828.14 mg/100 g)in fruit Libo-19 were the highest.