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中国精品科技期刊2020
陈玉子,谭乐和,刘启兵,等. 菠萝蜜多糖对小鼠肠道菌群的调节作用[J]. 食品工业科技,2022,43(24):189−196. doi: 10.13386/j.issn1002-0306.2022030215.
引用本文: 陈玉子,谭乐和,刘启兵,等. 菠萝蜜多糖对小鼠肠道菌群的调节作用[J]. 食品工业科技,2022,43(24):189−196. doi: 10.13386/j.issn1002-0306.2022030215.
CHEN Yuzi, TAN Lehe, LIU Qibing, et al. Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp on Gut Microbiota in Mice[J]. Science and Technology of Food Industry, 2022, 43(24): 189−196. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030215.
Citation: CHEN Yuzi, TAN Lehe, LIU Qibing, et al. Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp on Gut Microbiota in Mice[J]. Science and Technology of Food Industry, 2022, 43(24): 189−196. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030215.

菠萝蜜多糖对小鼠肠道菌群的调节作用

Modulatory Effect of Polysaccharide from Artocarpus heterophyllus Lam. (Jackfruit) Pulp on Gut Microbiota in Mice

  • 摘要: 为探究菠萝蜜多糖(JFP-Ps)对小鼠肠道菌群多样性及其结构的影响,将24只雄性昆明小鼠随机分为四组:对照组、JFP-Ps(50 mg/kg BW)组、JFP-Ps(100 mg/kg BW)组和JFP-Ps(200 mg/kg BW)组,连续灌喂2周后,收集新鲜小鼠粪便,提取粪便DNA扩增肠道菌群16S rRNA基因的V3~V4区,通过Illumina高通量测序技术研究菠萝蜜多糖对小鼠肠道菌群的调节作用。结果表明,JFP-Ps可通过调节拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)等肠道优势菌群的组成和结构,增加肠道菌群的多样性。通过属水平分析发现,JFP-Ps可增加拟杆菌属(Bacteroides)、异杆菌属(Allobaculum乳酸杆菌属(Lactobacillus)和副拟杆菌(Parabacteroides)等产SCFAs菌属的丰度,减少普雷沃菌属(Prevotella)等菌属丰度,发挥肠道微生物调节作用,改善宿主肠道微生态健康,研究结果为JFP-Ps在肠道益生产品的研发应用奠定理论基础。

     

    Abstract: The present study aimed to investigate the modulatory effect of polysaccharide from Artocarpus heterophyllus Lam. pulp (JFP-Ps) on the diversity and structure of gut microbiota in mice. 24 male Kunming mice were randomly divided into four groups: Control group, JFP-Ps (50 mg/kg BW) group, JFP-Ps (100 mg/kg BW) group and JFP-Ps (200 mg/kg BW) group. The colonic feces of mice were collected after treating with JFP-Ps in different doses for 2 weeks. Then the DNA was extracted and the V3~V4 region of the 16S rRNA gene of the intestinal flora was amplified. The regulatory effect of JFP-Ps on the gut microbiota of mice was studied using Illumina Miseq high-throughput sequencing technology. The results showed that Bacteroidetes and Firmicutes were the dominant intestinal flora, and JFP-Ps modulated the composition of Bacteroidetes and Firmicutes in comparison with the control group. At the genus level, the abundance of SCFAs-producing bacteria, including Bacteroides, Allobaculum, Lactobacillus and Parabacteroides were significantly increased in response to JFP-Ps treatment, but a significant decrease in Prevotella. These results indicated that JFP-Ps could improve the diversity of gut microbiota by regulating the composition and structure of dominant bacteria such as Bacteroidetes and Firmicutes. This study would provide a theoretical basis for JFP-Ps in intestinal prebiotics.

     

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