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中国精品科技期刊2020
单钱艺,沈岳明,张明兴,等. 正交试验优化超声波-复合酶水解法提取中华鳖油的工艺[J]. 食品工业科技,2022,43(22):256−264. doi: 10.13386/j.issn1002-0306.2022030035.
引用本文: 单钱艺,沈岳明,张明兴,等. 正交试验优化超声波-复合酶水解法提取中华鳖油的工艺[J]. 食品工业科技,2022,43(22):256−264. doi: 10.13386/j.issn1002-0306.2022030035.
SHAN Qianyi, SHEN Yueming, ZHANG Mingxing, et al. Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment[J]. Science and Technology of Food Industry, 2022, 43(22): 256−264. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030035.
Citation: SHAN Qianyi, SHEN Yueming, ZHANG Mingxing, et al. Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment[J]. Science and Technology of Food Industry, 2022, 43(22): 256−264. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022030035.

正交试验优化超声波-复合酶水解法提取中华鳖油的工艺

Optimization of Ultrasonic-Compound Enzyme Hydrolysis Method for Extracting Chinese Softshell Turtle Oil by Orthogonal Experiment

  • 摘要: 本研究以中华鳖加工副产物为原料,为提高中华鳖油提取率,使其高值化利用为生产企业提供新的利润源。采用超声波协同复合酶水解进行鳖油提取实验(酶解pH控制在7.5~8.5),利用单因素实验结合正交试验优化提取工艺参数,得到超声波-复合酶水解法提取最佳工艺条件:料液比为1:1.5(g/mL)、蛋白酶用量为2.4%、酶比例为1:1.5(中性蛋白酶:碱性蛋白酶)、酶水解时间2 h、酶水解温度65 ℃、超声功率180 W、超声时间30 min,所得到中华鳖油提取率为81.65%±0.62%。将中华鳖油进行精制后,测得其各项理化指标均符合国家水产行业标准(SC/T 3502-2016)精制鱼油一级标准。同时,脂肪酸组成分析得到精制后中华鳖油含有脂肪酸共计28种,其中饱和脂肪酸占总量23.81%,单不饱和脂肪酸占总量46.94%,多不饱和脂肪酸占总量26.38%,DHA及EPA含量占总量10.05%。

     

    Abstract: In this study, the by-products of Chinese softshell turtle processing were used as raw materials, which provided the possibility to improve the extraction efficiency of Chinese softshell turtle oil and make it high-value utilization to provide a new source of profit for production enterprises. The extraction experiment of turtle oil was carried out by using ultrasonic combined with complex enzymatic hydrolysis (The pH of enzymatic hydrolysis was controlled at 7.5~8.5), and the extraction parameters were optimized by single factor experiments combined with orthogonal experiment, and the optimal extraction process conditions of ultrasonic-complex enzymatic hydrolysis method were obtained: The ratio of solid to liquid was 1:1.5 (g/mL), the amount of protease was 2.4%, the ratio of enzyme was 1:1.5 (neutral protease:alkaline protease), the enzymatic hydrolysis time was 2 h, the enzymatic hydrolysis temperature was 65 ℃, the ultrasonic power was 180 W, and the ultrasonic time was 30 min. The extraction rate of Chinese softshell turtle oil was 81.65%±0.62%. After the Chinese softshell turtle oil was refined, its physical and chemical indicators were all in line with the national aquatic industry standard (SC/T 3502-2016) refined fish oil first-class standard. At the same time, fatty acid composition analysis showed that the refined Chinese softshell turtle oil contained a total of 28 kinds of fatty acids, of which saturated fatty acids accounted for 23.81% of the total, monounsaturated fatty acids accounted for 46.94% of the total, polyunsaturated fatty acids accounted for 26.38% of the total, DHA and EPA content accounted for 10.05% of the total.

     

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