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中国精品科技期刊2020
王艳,李虹佳,程美佳,等. 纳米金强化微波耦合脂肪酶催化油酸淀粉酯合成的研究[J]. 食品工业科技,2022,43(23):200−209. doi: 10.13386/j.issn1002-0306.2022020183.
引用本文: 王艳,李虹佳,程美佳,等. 纳米金强化微波耦合脂肪酶催化油酸淀粉酯合成的研究[J]. 食品工业科技,2022,43(23):200−209. doi: 10.13386/j.issn1002-0306.2022020183.
WANG Yan, LI Hongjia, CHENG Meijia, et al. AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester[J]. Science and Technology of Food Industry, 2022, 43(23): 200−209. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020183.
Citation: WANG Yan, LI Hongjia, CHENG Meijia, et al. AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester[J]. Science and Technology of Food Industry, 2022, 43(23): 200−209. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022020183.

纳米金强化微波耦合脂肪酶催化油酸淀粉酯合成的研究

AuNPs Enhanced Microwave Coupled Lipase Synthesis of Starch Oleate Ester

  • 摘要: 以预处理玉米淀粉和油酸为底物,纳米金固定后的南极假丝酵母脂肪酶(CAL@AuNPs)为催化剂,实现油酸淀粉酯的绿色高效制备。以油酸转化率为指标进行CAL@AuNPs制备条件的优化,取代度为指标探究游离酶、商品化固定化酶及纳米金固定化脂肪酶在微波辅助条件下的催化效率。结果表明,在40 ℃的条件下添加4 mL纳米金(14 nm)为载体对CAL固定6 h后,获得的CAL@AuNPs催化能力最强。在微波功率400 W,温度35 ℃的条件下CAL@AuNPs催化10 mL油酸和2 g淀粉反应40 min后,可以制得取代度最高为0.0259的油酸淀粉酯。与游离酶和商品化固定化酶相比,在微波辅助下,CAL@AuNPs催化能力强,能够有效缩短反应时间、提高产物取代度。

     

    Abstract: The nano-gold immobilized lipase (CAL@AuNPs) was prepared to synthesize starch oleate ester efficiently by using pre-treated corn starch and oleic acid as raw materials. Optimisation of the preparation conditions of CAL@AuNPs was carried out using oleic acid conversion rate as an indicator. The substitution degree was used as an indicator to investigate the catalytic efficiency of free enzyme, commercial immobilised enzyme and nanogold immobilised lipase under microwave-assisted conditions. The experimental results showed that the immobilized lipase with the strongest catalytic activity could be obtained by using AuNPs of 14 nm as carrier at 40 ℃ for 6 h. The starch oleate ester with the highest substitution degree of 0.0259 could be prepared by using CAL@AuNPs as catalyst under the condition of 400 W, 35 ℃ for 40 min. Compared with free enzyme, and commercial immobilized enzyme , the CAL@AuNPs could shorten reaction time and improve the degree of substitution.

     

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