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中国精品科技期刊2020
王琴飞,林立铭,张振文,等. 食用木薯块根及其制品中生氰糖苷检测方法的建立与应用[J]. 食品工业科技,2022,43(2):271−278. doi: 10.13386/j.issn1002-0306.2021040260.
引用本文: 王琴飞,林立铭,张振文,等. 食用木薯块根及其制品中生氰糖苷检测方法的建立与应用[J]. 食品工业科技,2022,43(2):271−278. doi: 10.13386/j.issn1002-0306.2021040260.
WANG Qinfei, LIN Liming, ZHANG Zhenwen, et al. Establishment and Application of A Method for Detecting the Cyanogenic Glycoside in Sweet Cassava Root and Its Products[J]. Science and Technology of Food Industry, 2022, 43(2): 271−278. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040260.
Citation: WANG Qinfei, LIN Liming, ZHANG Zhenwen, et al. Establishment and Application of A Method for Detecting the Cyanogenic Glycoside in Sweet Cassava Root and Its Products[J]. Science and Technology of Food Industry, 2022, 43(2): 271−278. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021040260.

食用木薯块根及其制品中生氰糖苷检测方法的建立与应用

Establishment and Application of A Method for Detecting the Cyanogenic Glycoside in Sweet Cassava Root and Its Products

  • 摘要: 生氰糖苷含量的高低对评估食用木薯块根及其制品的食用安全性及其重要。以木薯块根和木薯粉为试材,本研究建立了亚麻苦苷和百脉根苷2种生氰糖苷(Cyanogenic Glycosides,CNGs)的快速提取和高效液相色谱-蒸发光散射(HPLC-ELSD)检测的方法,分析了不同品种木薯块根、木薯粉及其制品中CNGs含量变化。结果表明,食用木薯块根及木薯粉中CNGs提取最佳的硫酸溶液浓度分别为0.025和0.100 mol/L;HPLC-ELSD检测方法可有效分离亚麻苦苷和百脉根苷,其浓度在4.1~820.0 mg/L和2.5~250.0 mg/L范围内线性相关性较好,相关系数(r)分别达到了0.9996和0.9993,检测限(LOD)分别为2.1和0.5 mg/kg,定量限(LOQ)分别为8.2和2.0 mg/kg;该方法重复性和样品稳定性的标准偏差(RSD)都低于5.0%;木薯块根和木薯粉中2种CNGs的平均加标回收率分别在89.3%~96.3%和107.5%~114.9%之间,重复间RSD小于3.4%。样品验证结果表明:不同的木薯品种CNGs含量差异较大;种植8~9个月的木薯制备木薯粉CNGs含量较低;蒸煮加工方式可有效清除木薯食品中CNGs。本方法操作简单、稳定性较好、准确度高,在食用木薯块根及其制品中CNGs的分析与评价等研究方面具有较强的应用前景。

     

    Abstract: The contents of cyanogenic glycosides (Cyanogenic Glycosides, CNGs) are very important to evaluate the safety of sweet cassava roots and their products. Using cassava roots and cassava products as test materials in this study, a method for rapid extraction and high performance liquid chromatography-evaporative light scattering (HPLC-ELSD) detection of two kinds of cyanogenic glycosides was established. The content of CNGs in different varieties of cassava roots, cassava flour, and their products were analyzed. The results showed that the optimal extraction concentrations of sulfuric acid solution for these two GNSs from cassava roots and flour were 0.025 and 0.100 mol/L, respectively. The HPLC-ELSD method was effective in the separation of linamarin and lotaustralin. The linear correlation was good in the range of 4.1 to 820.0 mg/L and 2.5 to 250.0 mg/L, which correlation coefficients (r) reached 0.9996 and 0.9993 respectively. The LOD was 2.1 and 0.5 mg/L, and the LOQ was 8.2 and 2.0 mg/kg, respectively. The relative standard deviation (RSD) of repeatability and sample stability are both less than 5.0%. The average recovery rates of two CNGs in cassava roots and cassava products were between 89.3%~96.3% and 107.5%~114.9%, respectively. And the RSD of the repeatability of the recovery was less than 3.4%. The results of sample verification showed that the content of CNGs in different cassava varieties were quite different, The content of CNGs was lower in cassava flour which was prepared from cassava grown for 8~9 months, and the cooking method could effectively remove CNGs in cassava food. This method was simple, stable and accurate, and had a strong application prospect in the analysis and evaluation of CNGs in sweet cassava roots and their products.

     

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