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中国精品科技期刊2020
柴美灵,李娜,乔宏萍,等. Box-Behnken法优化甘草多糖提取工艺及其体外抗氧化活性分析[J]. 食品工业科技,2021,42(23):192−200. doi: 10.13386/j.issn1002-0306.2021030248.
引用本文: 柴美灵,李娜,乔宏萍,等. Box-Behnken法优化甘草多糖提取工艺及其体外抗氧化活性分析[J]. 食品工业科技,2021,42(23):192−200. doi: 10.13386/j.issn1002-0306.2021030248.
CHAI Meiling, LI Na, QIAO Hongping, et al. Application of Box-Behnken Design for Ultrasonic-assisted Extraction of Glycyrrhiza Polysaccharide and Its Antioxidant Activity Analysis in vitro[J]. Science and Technology of Food Industry, 2021, 42(23): 192−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030248.
Citation: CHAI Meiling, LI Na, QIAO Hongping, et al. Application of Box-Behnken Design for Ultrasonic-assisted Extraction of Glycyrrhiza Polysaccharide and Its Antioxidant Activity Analysis in vitro[J]. Science and Technology of Food Industry, 2021, 42(23): 192−200. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021030248.

Box-Behnken法优化甘草多糖提取工艺及其体外抗氧化活性分析

Application of Box-Behnken Design for Ultrasonic-assisted Extraction of Glycyrrhiza Polysaccharide and Its Antioxidant Activity Analysis in vitro

  • 摘要: 本研究以传统炮制甘草为原材料,采用超声辅助水浴提取法,以粉碎粒径、液料比、超声温度和超声时间为考察因素,利用Box-Behnken响应面进行试验设计和曲面响应法对最佳甘草多糖提取条件进行优化,构建预测模型的二次多项式回归方程,并测定其体外抗氧化活性。结果表明,甘草多糖提取的最佳工艺条件为:粉碎粒径127 μm,液料比19.32 mL/g,超声温度65 ℃,超声时间30.2 min,多糖得率为10.867%。甘草粗提取物对DPPH·和ABTS+·均具有不同程度的清除作用,两种不同自由基的清除率与多糖提取物浓度之间存在一定量效关系,其IC50值分别2.80 mg/mL和1.96 mg/mL。本研究建立的模型可对甘草多糖得率进行分析和预测,并为甘草资源的开发和利用提供依据。

     

    Abstract: In the present paper, Glycyrrhiza was selected as raw materials, and ultrasonic-assisted water bath extraction method was used to investigate crushed particle size, liquid-material ratio, ultrasonic temperature and ultrasonic time. The ultrasonic-assisted extraction technology of polysaccharide from Glycyrrhiza was optimized by Box-Behnken design methodology and the quadratic polynomial regression equation of the prediction model was established, then the antioxidant activity of the polysaccharide in vitro was determined. According to the model regression analysis, the optimum conditions were as follows: Particle size of 127 μm, liquid-material ratio of 19.32 mL/g, ultrasonic temperature of 65 ℃ and ultrasonic time of 30.2 min. Under these conditions, the yield of polysaccharide reached 10.867%. Glycyrrhiza polysaccharide showed ability to scavenge both DPPH· and ABTS+· with IC50 values of 2.80 mg/mL and 1.96 mg/mL, respectively, and there was a certain dose-effect relationship between the scavenging rate of two different free radicals and the concentration of crude polysaccharide extract. The model established in this study can analyze and predict the yield of Glycyrrhiza polysaccharide to determine the natural antioxidant activity, which can provide an experimental basis for the development and utilization of Glycyrrhiza resources.

     

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