• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
刘敏敏,谢小霞,陈俏,等. 液相色谱-质谱联用法(HPLC-MS/MS)快速测定凉茶中的5种罂粟壳类生物碱[J]. 食品工业科技,2021,42(23):266−272. doi: 10.13386/j.issn1002-0306.2021020209.
引用本文: 刘敏敏,谢小霞,陈俏,等. 液相色谱-质谱联用法(HPLC-MS/MS)快速测定凉茶中的5种罂粟壳类生物碱[J]. 食品工业科技,2021,42(23):266−272. doi: 10.13386/j.issn1002-0306.2021020209.
LIU Minmin, XIE Xiaoxia, CHEN Qiao, et al. Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS[J]. Science and Technology of Food Industry, 2021, 42(23): 266−272. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020209.
Citation: LIU Minmin, XIE Xiaoxia, CHEN Qiao, et al. Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS[J]. Science and Technology of Food Industry, 2021, 42(23): 266−272. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020209.

液相色谱-质谱联用法(HPLC-MS/MS)快速测定凉茶中的5种罂粟壳类生物碱

Rapid Determination of Five Alkaloids in Poppy Shell in Herbal Tea by HPLC-MS/MS

  • 摘要: 目的:建立液相色谱-质谱联用法(HPLC-MS/MS)快速检测凉茶中非法添加的吗啡、可待因、蒂巴因、罂粟碱、那可丁等5种罂粟壳类生物碱。方法:采用Agilent Poroshell 120 EC-C18(2.1 mm×100 mm,2.7 μm)色谱柱分离,含0.1%乙酸的5 mmol/L乙酸铵溶液-甲醇为流动相梯度洗脱,在电喷雾离子源正离子模式下,采用多反应监测(MRM)方式测定,外标法定量。结果:吗啡、可待因的线性范围为2.5~100 ng/mL,蒂巴因、罂粟碱、那可丁的线性范围为0.5~20 ng/mL,5种罂粟壳类生物碱的线性相关系数均大于0.995(R>0.995)。检出限吗啡、可待因为0.8 μg/g,罂粟碱、蒂巴因、那可丁为0.2 μg/g。5种生物碱成分的3个添加水平在液体基质中回收率为84.5%~105.1%,RSD不大于6.3%(n=6),固体基质中回收率为72.5%~112.3%,RSD不大于7.1%(n=6)。应用方法对150批凉茶样品进行了抽检,其中6批检出。结论:该方法操作简便,准确可靠,适用于快速分析测定凉茶中的5种罂粟壳类成分。

     

    Abstract: Objective: A HPLC-MS/MS method was developed for rapid determination of five alkaloids in Poppy Shell in herbal tea, including morphine, codeine, thebaine, papaverine, and narcotine. Method: Agilent Poroshell 120 EC-C18(2.1 mm×100 mm, 2.7 μm)was applied with the mobile phase consisting of 5 mmol/L ammonium acetate containing 0.1 % acetic acid-methanol by gradient elution. The electrospray ion source was operated in the positive ion mode using the multi-reaction monitoring (MRM) method, and the results were quantified by the external standard method. Result: The concentration range was 2.5~100 ng/mL in morphine and codeine, 0.5~20 ng/mL in the others. The correlation coefficients (R) were all above 0.995. The detection limit was 0.8 μg/g in morphine and codeine, 0.2 μg/g in the others. The average recoveries ranged from 84.5% to 105.1% in liquid samples, with relative stand deviations (RSDs) all below 6.3% (n=6), ranged from 72.5% to 112.3% in solid samples, with relative stand deviations (RSDs) all below 7.1% (n=6). 150 Batches of samples bought from markets were screened, and 6 batches showed positive for these analytes. Conclusion: This method was simple, accurate, could be used for the determination of five alkaloids in Poppy Shell added in herbal tea.

     

/

返回文章
返回