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中国精品科技期刊2020
王文平,熊英梅,陈赛浙,等. 不同地区米酒曲细菌多样性解析及表型预测[J]. 食品工业科技,2021,42(20):134−139. doi: 10.13386/j.issn1002-0306.2021020035.
引用本文: 王文平,熊英梅,陈赛浙,等. 不同地区米酒曲细菌多样性解析及表型预测[J]. 食品工业科技,2021,42(20):134−139. doi: 10.13386/j.issn1002-0306.2021020035.
WANG Wenping, XIONG Yingmei, CHEN Saizhe, et al. Analysis of Bacterial Diversity and Prediction of Phenotype in Rice Wine Koji from Different Regions[J]. Science and Technology of Food Industry, 2021, 42(20): 134−139. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020035.
Citation: WANG Wenping, XIONG Yingmei, CHEN Saizhe, et al. Analysis of Bacterial Diversity and Prediction of Phenotype in Rice Wine Koji from Different Regions[J]. Science and Technology of Food Industry, 2021, 42(20): 134−139. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021020035.

不同地区米酒曲细菌多样性解析及表型预测

Analysis of Bacterial Diversity and Prediction of Phenotype in Rice Wine Koji from Different Regions

  • 摘要: 以广西省南宁地区和湖北省孝感地区的米酒曲为研究对象,在采用Illumina MiSeq高通量测序技术对其16S rRNA V3~V4区进行测序的基础上,结合生物信息学和多元统计学手段对2个地区米酒曲细菌多样性进行了解析,同时对其细菌类群表型进行了预测。结果表明:南宁地区米酒曲细菌类群的香农指数显著低于孝感地区(P<0.05);基于加权UniFrac距离的主坐标分析显示2个地区样本在空间排布上呈现明显的分离趋势;多元方差分析结果表明2个地区米酒曲细菌群落结构差异显著(P<0.05);南宁地区米酒曲细菌类群以Lactobacillus(乳杆菌属)为主,平均相对含量为62.03%,而孝感地区以Weissella(魏斯氏菌属)为主,平均相对含量为50.14%。表型预测结果表明,南宁地区米酒曲中革兰氏阳性菌含量高度显著偏高(P<0.01),而细菌类群的氧化胁迫耐受、生物膜形成、革兰氏阴性、致病潜力和兼性厌氧特性高度显著偏低(P<0.01)。由此可见,不同地区米酒曲的细菌类群存在一定的差异。

     

    Abstract: A rice wine koji collected from Nanning of Guangxi province and Xiaogan of Hubei province were used to sequence its 16S rRNA V3~V4 region based on Illumina Miseq high-throughput sequencing technology, combined with bioinformatics and multivariate statistical methods to analysis the bacterial diversity of rice wine koji in the two regions, while the bacterial taxa phenotypes were predicted. Results showed that, the Shannon index of the bacterial taxa in the rice wine koji from Nanning area was significantly lower than that of the Xiaogan area(P<0.05). Principal coordinate analysis based on weighted UniFrac distances showed a clear separation trend between the samples from the 2 regions in spatial arrangement. Multivariate analysis of variance indicated that the bacterial community structures of the rice wine koji in the 2 regions were significantly different(P<0.05). The bacterial taxa of rice wine koji in Nanning area were mainly Lactobacillus with an average relative abundance of 62.03%, while Weissella was predominant in the Xiaogan area with an average relative abundance of 50.14%. Phenotypic prediction showed that rice wine koji from Nanning area exhibited a significantly higher(P<0.01) abundance of gram-positive bacteria, while a significantly lower(P<0.01) abundance of oxidative stress tolerance, biofilm formation, gram negative, pathogenic potential and facultative anaerobic characteristics. It could be concluded that there were some differences in the bacterial taxa of rice wine koji from different regions.

     

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