Abstract: The aim of this study was to establish a method for the determination of dihydroquercetin, a new food additive, in dairy products by High-performance liquid chromatography. The dihydroquercetin was extracted by acetonitrile /water solution, then was purified by extraction column with Polystyrene / divinylbenzene Copolymer (HLB or PLS). After separation by C₁₈ reversed-phase chromatographic column, the content of dihydroquercetin was detected by the UV detector detection. The results showed that the linear range of dihydroquercetin was 0.5~20 μg/mL, the linear correlation coefficient was 0.9997, the recovery range of different kinds of samples was 86.0%~101%, the precision RSD was 2.06% (n=6).The method has good reproducibility and high accuracy. It can provide reference for the determination of dihydroquercetin in food and the establishment of national standard.