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中国精品科技期刊2020
范广宇,唐秀,张琳,等. 高效液相色谱-串联质谱法测定鲆鲽类水产品中喹诺酮的不确定度评定[J]. 食品工业科技,2021,42(20):248−254. doi: 10.13386/j.issn1002-0306.2021010077.
引用本文: 范广宇,唐秀,张琳,等. 高效液相色谱-串联质谱法测定鲆鲽类水产品中喹诺酮的不确定度评定[J]. 食品工业科技,2021,42(20):248−254. doi: 10.13386/j.issn1002-0306.2021010077.
FAN Guangyu, TANG Xiu, ZHANG Lin, et al. Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(20): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010077.
Citation: FAN Guangyu, TANG Xiu, ZHANG Lin, et al. Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2021, 42(20): 248−254. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010077.

高效液相色谱-串联质谱法测定鲆鲽类水产品中喹诺酮的不确定度评定

Uncertainty Evaluation of Determination of Quinolones in Flatfish by High Performance Liquid Chromatography-Tandem Mass Spectrometry

  • 摘要: 采用高效液相色谱-串联质谱法测定鲆鲽类水产品中的氧氟沙星、培氟沙星、诺氟沙星和洛美沙星残留量。参照标准SN/T 1751.2-2007 《进出口动物源食品中喹诺酮类药物残留量检测方法 第2部分:液相色谱-质谱/质谱法》建立了数学模型,分析不确定度的来源并评定。当鲆鲽类水产品中氧氟沙星含量为9.26 μg/kg时,其扩展不确定度为1.04 μg/kg(k=2);培氟沙星含量为10.08 μg/kg,其扩展不确定度为1.09 μg/kg(k=2);诺氟沙星含量为9.73 μg/kg时,其扩展不确定度为1.34 μg/kg(k=2);当洛美沙星含量为9.57 μg/kg,其扩展不确定度为0.99 μg/kg(k=2)。结果表明,不确定度主要来自标准曲线拟合、高效液相色谱-串联质谱仪和标准溶液配制。

     

    Abstract: A method for the determination of ofloxacin, pefloxacin, norfloxacin and lomefloxacin in flatfish by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established. Accoding to SN/T 1751.2-2007 Determination of Multi-residues of Quinolones in Food of Animal Origin for Import and Export-Part 2: LC-MS/MS Method, a mathematical model for uncertainty evaluation was established and the sources of uncertainty were analysed. The expanded uncertainty was 1.04, 1.09, 1.34 and 0.99 μg/kg(k=2) for ofloxacin, pefloxacin, norfloxacin and lomefloxacin of contents of 9.26, 10.08, 9.73 and 9.57 μg/kg in flatfish, respectively. As a result, the fitting of the standard curve, the high performance liquid chromatography-tandem mass spectrometry and the preparation of standard solution were the main sources of uncertainty.

     

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