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中国精品科技期刊2020
牛鹤丽. 响应面法优化大蓟根总黄酮纯化工艺及其纯化前、后的抑菌活性比较[J]. 食品工业科技,2021,42(19):211−217. doi: 10.13386/j.issn1002-0306.2021010058.
引用本文: 牛鹤丽. 响应面法优化大蓟根总黄酮纯化工艺及其纯化前、后的抑菌活性比较[J]. 食品工业科技,2021,42(19):211−217. doi: 10.13386/j.issn1002-0306.2021010058.
NIU Heli. Optimization of Purification Process of Total Flavonoids from Roots of Cirsium japonicum DC. with Macroporous Resin by Response Surface Analysis and Comparison of Its Anti-bacterial Activity before and after Purification[J]. Science and Technology of Food Industry, 2021, 42(19): 211−217. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010058.
Citation: NIU Heli. Optimization of Purification Process of Total Flavonoids from Roots of Cirsium japonicum DC. with Macroporous Resin by Response Surface Analysis and Comparison of Its Anti-bacterial Activity before and after Purification[J]. Science and Technology of Food Industry, 2021, 42(19): 211−217. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010058.

响应面法优化大蓟根总黄酮纯化工艺及其纯化前、后的抑菌活性比较

Optimization of Purification Process of Total Flavonoids from Roots of Cirsium japonicum DC. with Macroporous Resin by Response Surface Analysis and Comparison of Its Anti-bacterial Activity before and after Purification

  • 摘要: 在单因素实验的基础上,利用响应面试验优化大孔树脂纯化大蓟根黄酮提取物工艺,并比较纯化前、后的抑菌活性。通过静态吸附-洗脱实验,考察不同类型大孔树脂对大蓟根黄酮化合物的吸附与解吸性能,选择最佳吸附树脂型号后,采用动态吸附-洗脱实验确定最佳纯化工艺条件,另通过“滤纸片法”考察纯化前、后的黄酮化合物对大肠杆菌、枯草杆菌和金黄色葡萄球菌的抑菌活性。结果表明,大蓟根黄酮提取物的最佳纯化工艺条件为:质量浓度为2.96 mg/mL、pH4.97的黄酮提取液70 mL,以1.0 mL/min流速上样至D101型树脂后,经180 mL体积分数70.2%乙醇溶液,以1.0 mL/min流速洗脱,在该条件下总黄酮的回收率为69.8%,纯度为67.4%。体外抑菌试验结果表明,与提取物相比,纯化后的黄酮化合物对三种受试菌的抑制圈直径均有显著性增大(P<0.05),抑菌活性更好。

     

    Abstract: The purification process of total flavonoids that extract from roots of Cirsium japonicum DC. with macroporous resin was optimized by response surface experiments based on single factor tests, and anti-bacterial activity of flavonoid were compared before and after purification. The static adsorption and desorption performances of different types macroporous resin were observed by static adsorption-desorption experiment for confirmed the best type resin. Then, the best purified process was determined by dynamic adsorption-desorption experiment. Meanwhile, the resistant abilities against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis of two products were detected by filtering paper method. The results showed that D101 was the best purified resin. The optimal parameters for purification were as follows: the sample concentration of 2.96 mg/mL, the sample pH value of 4.97, the sample flow rate of 1.0 mL/min, ethanol concentration of 70.2% eluting agent volume of 180 mL, and desorption rate of 1.0 mL/min. Under these conditions, total flavonoids had a recovery rate of 69.8% and a purity of 67.4%. The results of anti-bacterial test in vitro showed that compared with extract, the diameter of inhibition zone which flavonoid after purification inhibited three tested bacteria were obviously all increasing (P<0.05). Therefore, its anti-bacterial activity was better.

     

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