Abstract: To investigate the effects of Altay lipid and its extract on liver lipid level and oxidative stress in mice, 84 Kunming mice were randomly divided into two batches of 20 days and 40 days, 7 groups each (n=6): blank group (basic feed feeding), rapeseed oil group, Altay sheep buttocks oil group, 2 ℃ solid oil group, 12 ℃ solid oil group, 22 ℃ solid oil group, 32 ℃ solid oil group, experimental oil group fed 0.15 mL/10 g of basic feed to mice, the liver index of mice was measured, pathology were observed, liver lipid level and oxidative stress index were determined according to the kit specification. The results showed that there was no significant effect on liver index of mice with Altay sheep buttocks fat and its extract (P>0.05), no pathological changes in liver tissue, compared with rapeseed oil, the Altay sheep buttocks fat extracts could effectively reduce the liver lipid level of mice, and the effect of 2 ℃ solid oil group was more significant (P<0.05). At 40 days, the MDA content of the rapeseed oil group was significantly higher than that of the other groups (P<0.05); Compared with the blank group, 32 ℃ solid fat group SOD, CAT, GSH-Px enzyme activities and T-AOC were significantly increased (P<0.05), while the MDA content was significantly decreased (P<0.05). With the increase of intake time, liver index of rapeseed oil group increased by 8.21% significantly, the TC level of the Altay sheep buttocks oil group decreased significantly, and the HDL-C level increased significantly, the MDA content of 32 ℃ solid fat group decreased significantly and the SOD activity increased 30.85% significantly. Different Altay sheep buttocks fat extracts has different effects on the liver lipid levels and oxidative stress in mice. Compared with rapeseed oil, Altay sheep buttocks oil is more beneficial to human health. The 2 ℃ solid oil in the Altay sheep buttocks fat extracts can effectively control the increase of liver lipids in mouse, and the 32 ℃ solid oil enhances antioxidant enzyme activity and reduces oxidation damage effect is better.