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中国精品科技期刊2020
张双虹,刘孟涛,陆婉瑶,等. Illumina Miseq高通量测序方法对袋装番茄酱微生物多样性分析[J]. 食品工业科技,2021,42(18):119−125. doi: 10.13386/j.issn1002-0306.2020120254.
引用本文: 张双虹,刘孟涛,陆婉瑶,等. Illumina Miseq高通量测序方法对袋装番茄酱微生物多样性分析[J]. 食品工业科技,2021,42(18):119−125. doi: 10.13386/j.issn1002-0306.2020120254.
ZHANG Shuanghong, LIU Mengtao, LU Wanyao, et al. Analysis of Microbial Diversity in the Bagged Tomato Sauce by Illumina Miseq High Throughput Sequencing[J]. Science and Technology of Food Industry, 2021, 42(18): 119−125. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120254.
Citation: ZHANG Shuanghong, LIU Mengtao, LU Wanyao, et al. Analysis of Microbial Diversity in the Bagged Tomato Sauce by Illumina Miseq High Throughput Sequencing[J]. Science and Technology of Food Industry, 2021, 42(18): 119−125. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120254.

Illumina Miseq高通量测序方法对袋装番茄酱微生物多样性分析

Analysis of Microbial Diversity in the Bagged Tomato Sauce by Illumina Miseq High Throughput Sequencing

  • 摘要: 目的:研究袋装番茄酱的微生物多样性及差异。方法:采集番茄酱样品,采用Illumina Miseq-高通量测序方法对产自新疆地区的番茄酱进行测定,并结合计数方法对胀袋番茄酱中菌株进行计数和鉴定。结果:胀袋番茄酱中的腐败微生物为乳杆菌属微生物,正常番茄酱中鉴定出的微生物包括乳杆菌属、伯克氏菌属、劳尔氏菌属、草螺菌属、慢生根瘤菌等主要存在于植物和土壤中的细菌。结论:本研究为了解番茄酱制品的微生物多样性提供了一定的参考。

     

    Abstract: Objective: The microbial community structures of bagged tomato sauce were evaluated. Methods: Tomato sauce samples were collected from Xinjiang Uygur Autonomous region. Samples after collected were analyzed by Illumina Miseq sequencing, and the microorganisms in swell bagged tomato sauce were counted and identified by combining counted method. Results: The Lactobacillus was the main spoilage microorganisms in the bagged tomato sauce. Microorganisms identified in normal tomato sauce included Lactobacillus, Burkholderia, Ralstonia, Herbaspirillum, Bradyrhizobium and other bacteria mainly existing in plants and soil. Conclusion: This study would provide insights into the microbial diversity of tomato sauce.

     

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