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中国精品科技期刊2020
杜月梅,刘云帆,秦菲,等. 鱼油对顺铂损伤HEK293细胞及A549细胞的影响[J]. 食品工业科技,2022,43(23):347−352. doi: 10.13386/j.issn1002-0306.2020120173.
引用本文: 杜月梅,刘云帆,秦菲,等. 鱼油对顺铂损伤HEK293细胞及A549细胞的影响[J]. 食品工业科技,2022,43(23):347−352. doi: 10.13386/j.issn1002-0306.2020120173.
DU Yuemei, LIU Yunfan, QIN Fei, et al. Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells[J]. Science and Technology of Food Industry, 2022, 43(23): 347−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120173.
Citation: DU Yuemei, LIU Yunfan, QIN Fei, et al. Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells[J]. Science and Technology of Food Industry, 2022, 43(23): 347−352. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120173.

鱼油对顺铂损伤HEK293细胞及A549细胞的影响

Effects of Fish Oil Combined with Cisplatin on HEK293 Cells and A549 Cells

  • 摘要: 目的:探讨鱼油对顺铂(cis-dichlorodiamineplatinum (Ⅱ),CDDP)抗A549人肺腺癌细胞活性的影响及对CDDP损伤HEK293细胞的保护作用。方法:体外培养A549和HEK293细胞,将细胞分为对照组、CDDP组、鱼油组、鱼油+CDDP组;分别用不同浓度的鱼油对细胞进行预处理,同时建立CDDP组、鱼油+CDDP组CDDP 损伤模型,CCK-8法检测细胞活力,试剂盒测定HEK293细胞中丙二醛(MDA)含量,二还原型谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD)的活力等指标。结果:4~16 mg/L浓度的鱼油能够显著抑制CDDP造成的人胚肾HEK293细胞毒性(P<0.05),其中4 mg/L浓度的鱼油保护效果最佳,且此浓度的鱼油能够显著抑制CDDP(20 mg/L)引起的GSH含量降低以及MDA含量升高(P<0.05)。当鱼油浓度≥32 mg/L时显著增强了CDDP对A549细胞的抑制效果(P<0.05)。结论:一定浓度范围内,鱼油对CDDP致人胚肾细胞HEK293损伤具有一定的保护作用。低浓度鱼油对CDDP 所致A549细胞的抑制作用没有明显的影响,高浓度的鱼油增强了CDDP 的抗癌效果。

     

    Abstract: Objective: To explore the effect of fish oil on the activity of cis-dichlorodiamineplatinum (Ⅱ) (CDDP) against A549 human lung adenocarcinoma cells and its protective effect on HEK293 cells damaged by CDDP. Methods: A549 and HEK293 cells were cultured in vitro, and the cells were divided into control group, CDDP group, fish oil group, fish oil+CDDP group; the cells were pretreated with different concentrations of fish oil, and CDDP injury models of CDDP group and fish oil+CDDP group were established at the same time. Cell viability was detected by CCK-8 method, and the kits were used to measure malondialdehyde (MDA) content, direduced glutathione (GSH) content, and superoxide dismutase (SOD) activity in HEK293 cells. Results: Fish oil at a concentration of 4~16 mg/L could significantly inhibit the cytotoxicity of HEK293 cells caused by CDDP (P<0.05). Fish oil at a concentration of 4 mg/L had the best protective effect, and fish oil at this concentration could significantly inhibit CDDP (20 mg/L) decreased GSH content and increased MDA content (P<0.05). When the fish oil concentration was ≥32 mg/L, the inhibitory effect of CDDP on A549 cells was significantly enhanced (P<0.05). Conclusion: Within a certain concentration range, fish oil has a certain protective effect on CDDP-induced damage to HEK293 of human embryonic kidney cells. Low-concentration fish oil has no obvious effect on the inhibitory effect of CDDP-induced A549 cells, and high-concentration fish oil enhances the anti-cancer effect of CDDP.

     

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