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中国精品科技期刊2020
周静萍,刘伟,杨春燕,等. 双菌微囊化发酵耦合泡沫分离强化乳链菌肽生产的工艺[J]. 食品工业科技,2021,42(17):179−186. doi: 10.13386/j.issn1002-0306.2020120101.
引用本文: 周静萍,刘伟,杨春燕,等. 双菌微囊化发酵耦合泡沫分离强化乳链菌肽生产的工艺[J]. 食品工业科技,2021,42(17):179−186. doi: 10.13386/j.issn1002-0306.2020120101.
ZHOU Jingping, LIU Wei, YANG Chunyan, et al. Strengthening Nisin Production by Using the Coupling Technology of Foam Fractionation and Microcapsule Cells Immobilizing Two Strains Fermentation[J]. Science and Technology of Food Industry, 2021, 42(17): 179−186. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120101.
Citation: ZHOU Jingping, LIU Wei, YANG Chunyan, et al. Strengthening Nisin Production by Using the Coupling Technology of Foam Fractionation and Microcapsule Cells Immobilizing Two Strains Fermentation[J]. Science and Technology of Food Industry, 2021, 42(17): 179−186. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020120101.

双菌微囊化发酵耦合泡沫分离强化乳链菌肽生产的工艺

Strengthening Nisin Production by Using the Coupling Technology of Foam Fractionation and Microcapsule Cells Immobilizing Two Strains Fermentation

  • 摘要: 本研究拟通过共培养乳酸链球菌与酿酒酵母解除产物抑制效应,并将泡沫分离耦合于微胶囊固定细胞发酵强化乳链菌肽(Nisin)的生产。采用液芯微胶囊固定乳酸链球菌和酿酒酵母细胞,并对海藻酸钠和壳聚糖浓度进行优化。结果表明,以乳糖为底物碳源,乳酸链球菌与酿酒酵母的初始接种比例102:1时,共培养发酵液中Nisin效价高达3436.3 IU/mL。海藻酸盐-壳聚糖-海藻酸盐(ACA)液芯微胶囊的制备条件为海藻酸钠浓度15 g/L和壳聚糖浓度5.5 g/L。在微胶囊固定细胞发酵与泡沫分离耦合时间为21 h,分布器孔径180 μm,气体体积流率40 mL/min条件下,Nisin的富集比和回收率分别为5.8和63.1%,总效价为3973.2 IU/mL。本研究建立的双菌微囊化发酵-泡沫分离耦合工艺能够有效解除产物抑制和菌体细胞损失,为高密度、连续发酵生产Nisin奠定了理论基础。

     

    Abstract: This work was aimed at strengthening Nisin production by coupling foam fractionation with microcapsule cells fermentation, in which Lactococcus lactis subsp.lactis (L.lactis) and Saccharomyces cerevisiae (S.cerevisiae) were co-cultivated to relieve the product inhibition from lactic acid. The concentrations of sodium alginate and chitosan were optimized to prepare liquid-core microcapsules for immobilizing L.lactis and S.cerevisiae. Results indicated that the Nisin titer in co-cultivation fermentation broth was as high as 3436.3 IU/mL by using lactose as carbon source and setting initial inoculation ratio of L.lactis and S.cerevisiae at 102:1. The optimal preparation conditions of sodium alginate/chitosan/sodium alginate (ACA) liquid-core microcapsules were sodium alginate concentration of 15 g/L and chitosan concentration of 5.5 g/L. Under the suitable conditions of foam fractionation coupled with microcapsule cells fermentation after cultivation for 21 h, pore diameter of gas distributor 180 μm and volumetric air flow rate 40 mL/min, the enrichment ration and recovery percentage of Nisin reached 5.8 and 63.1%, respectively. The total yield of Nisin in fermentation broth was 3973.2 IU/mL. The coupling technology of microcapsule cells immobilizing two strains fermentation and foam fractionation could effectively overcome the obstacles of product inhibition and strain cell loss, thus facilitating the high-density and continuous fermentation for Nisin production.

     

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