Abstract: Objective: The present study was carried on to assess the immunoregulatory effects of complexes obtained by any 3 polysaccharides from five kinds of different edible fungi polysaccharides, including Tremella fuciformis polysaccharide, Poriacocos polysaccharide, Lentinan, Hericium edodes polysaccharide and Dictyophora indusiata polysaccharide. Methods: Effects of different concentrations of complexes on the proliferation of RAW264.7 were explored using cell counting kit (CCK)-8, the levels of tumor necrosis factor (TNF)-α in the culture medium were detected after complexes treatment by enzyme-linked immunosorbent assay (ELISA). In addition, flow cytometry was employed to analyze the effect of complexes on RAW 264.7 phagocytic capacity. Results: Compared with normal control group, ten kinds of complexes exerted better positive effects on the proliferation of macrophages. Meanwhile, the complexes obviously enhanced phagocytic capacity as well as increased the TNF-α level. Furthermore, 10 µg/mL complex of Tremella fuciformis polysaccharide, Poriacocos polysaccharide and Dictyophora indusiata polysaccharide exhibited the best effect on the proliferation of macrophages, 160 µg/mL complex of Tremella fuciformis polysaccharide, Poriacocos polysaccharide and Lentinan showed the best effect on enhancing both secretion of TNF-α and phagocytic ability of RAW264.7. Conclusion: The complexes of polysaccharides from these five kinds of edible fungi displayed excellent effect on regulating immunity of macrophage RAW 264.7. The results could provide data reference for the development of immunomodulatory polysaccharide products.