• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
叶辉宇,李强明,张玉英,等. 灵芝子实体不同提取物对小鼠睡眠改善作用的比较[J]. 食品工业科技,2021,42(17):350−356. doi: 10.13386/j.issn1002-0306.2020090275.
引用本文: 叶辉宇,李强明,张玉英,等. 灵芝子实体不同提取物对小鼠睡眠改善作用的比较[J]. 食品工业科技,2021,42(17):350−356. doi: 10.13386/j.issn1002-0306.2020090275.
YE Huiyu, LI Qiangming, ZHANG Yuying, et al. Compared with the Effect of Sleep Improvement by Different Extracts from Ganoderma Lucidum Fruit Bodies in Mice[J]. Science and Technology of Food Industry, 2021, 42(17): 350−356. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090275.
Citation: YE Huiyu, LI Qiangming, ZHANG Yuying, et al. Compared with the Effect of Sleep Improvement by Different Extracts from Ganoderma Lucidum Fruit Bodies in Mice[J]. Science and Technology of Food Industry, 2021, 42(17): 350−356. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020090275.

灵芝子实体不同提取物对小鼠睡眠改善作用的比较

Compared with the Effect of Sleep Improvement by Different Extracts from Ganoderma Lucidum Fruit Bodies in Mice

  • 摘要: 目的:观察灵芝子实体水提物、醇提物和多糖对睡眠的改善作用并初步探讨作用机制。方法:将ICR小鼠随机分为正常对照组、阳性对照组(罗通定片,15 mg/kg)、灵芝子实体水提物高剂量组(GLW-H,154.2 mg/kg)和低剂量组(GLW-L,77.1 mg/kg)、灵芝子实体醇提物高剂量组(GLE-H,125.4 mg/kg)和低剂量组(GLE-L,62.7 mg/kg)及灵芝子实体多糖高剂量组(GLP-H,31.8 mg/kg)和低剂量组(GLP-L,15.9 mg/kg),连续灌胃30 d。末次灌胃后分别进行直接睡眠实验、延长戊巴比妥钠睡眠时间实验、戊巴比妥钠阈下剂量催眠实验和巴比妥钠睡眠潜伏期实验,并测定小鼠脑内γ-氨基丁酸(γ-aminobutyric acid,GABA)、5-羟色胺(5-hydroxytryptamine,5-HT)和多巴胺(Dopamine,DA)水平。结果:灵芝子实体水提物、醇提物和多糖对小鼠体重、脏器指数均无影响,各实验组小鼠给药60 min内均无出现直接睡眠现象。与正常对照组比较,GLW-H、GLE-H、GLE-L、GLP-H组均能显著延长戊巴比妥钠睡眠时间(P<0.05),以及缩短巴比妥钠睡眠潜伏期(P<0.05),小鼠脑内GABA、5-HT含量上升(P<0.05),DA含量变化不显著(P>0.05)。GLE-H组较正常对照组组能显著增加戊巴比妥钠阈下剂量催眠实验小鼠睡眠率(P<0.05)。结论:灵芝子实体水提物、醇提物、多糖对小鼠均具有改善睡眠作用,在相同生药剂量下总体趋势为:灵芝子实体醇提物>灵芝子实体水提物>灵芝子实体多糖,其改善睡眠作用可能与上调小鼠脑内GABA、5-HT含量有关。

     

    Abstract: Objective: The aim of this work was to investigate the effects of Ganoderma lucidum fruit body water extract (GLW), ethanol extract (GLE) and polysaccharide (GLP) on sleep improvement in mice, and preliminarily clarify the underlying mechanism. Methods: ICR mice were randomly divided into normal group, positive group (rotundine, 15 mg/kg), GLW (154.2 mg/kg, GLW-H group; 77.1 mg/kg, GLW-L group), GLE group (125.4 mg/kg, GLE-H group; 62.7 mg/kg, GLE-L group), GLP (31.8 mg/kg, GLP-H group; 15.9 mg/kg, GLP-L group) for 30 days. After the last oral administration, direct hypnosis experiment, prolonging pentobarbital sodium sleep time experiment, subthreshold dose of pentobarbital sodium hypnotic experiment and barbital sodium sleep incubation period experiment were conducted. The level of γ-aminobutyric acid (GABA), 5-hydroxytryptamine (5-HT) and dopamine (DA) in brain of mice were measured. Result: Administration with GLW, GLE and GLP did not affect the body weight and viscera index of mice. Administration with GLW-H, GLE-H, GLE-L and GLP-H was no influence of direct sleep in each experimental group within 60 minutes, but it could significantly prolong the sleep time of mice treated with pentobarbital sodium (P<0.05), decrease the sleep incubation period of mice treated with barbital sodium (P<0.05) and increase the level of GABA and 5-HT in brain (P<0.05).But the level of DA was not affected (P>0.05). Additionally, administration with GLE-H could significantly increase the sleep rate of mice treated with subthreshold dosage of pentobarbital sodium (P<0.05). Conclusion: GLW, GLE and GLP could promote sleep of mice via regulating the level of GABA and 5-HT in brain. Under the same dosage of crude drug, the general trend improving sleep of different extracts from Ganoderma lucidum fruit body was as follows: alcohol extract> water extract> polysaccharide.

     

/

返回文章
返回