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中国精品科技期刊2020
张瑛毓,普布多吉,卢聪,等. 基于黄嘌呤氧化酶活性抑制和斑马鱼高尿酸血症模型的降尿酸功效食药材筛选[J]. 食品工业科技,2021,42(12):334−339. doi: 10.13386/j.issn1002-0306.2020080220.
引用本文: 张瑛毓,普布多吉,卢聪,等. 基于黄嘌呤氧化酶活性抑制和斑马鱼高尿酸血症模型的降尿酸功效食药材筛选[J]. 食品工业科技,2021,42(12):334−339. doi: 10.13386/j.issn1002-0306.2020080220.
ZHANG Yingyu, PUBU Duoji , LU Cong, et al. Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model[J]. Science and Technology of Food Industry, 2021, 42(12): 334−339. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080220.
Citation: ZHANG Yingyu, PUBU Duoji , LU Cong, et al. Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model[J]. Science and Technology of Food Industry, 2021, 42(12): 334−339. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080220.

基于黄嘌呤氧化酶活性抑制和斑马鱼高尿酸血症模型的降尿酸功效食药材筛选

Screening of Uric Acid-lowering Food and Medicinal Materials Based on Inhibiting Xanthine Oxidase Activity and Zebrafish Hyperuricemia Model

  • 摘要: 结合体外(黄嘌呤氧化酶活性抑制)和体内(斑马鱼高尿酸血症模型)方法,对15种食药材乙醇粗提物的降尿酸活性进行筛选。体外实验设置空白组、酶反应组、抑制剂组、对照组,酶标仪295 nm测定吸光度,计算15种食药材对黄嘌呤氧化酶(XOD)活性的抑制率;体内实验随机选取受精后第5 d(5dpf)的斑马鱼,设置空白组、模型组(200 μmol/L PO+10 μmol/L XSS)、食药材给药组(200 μmol/L PO+10 μmol/L XSS+不同浓度提取物)、阳性对照组(200 μmol/L PO+10 μmol/L XSS+APL 2 mmol/L),水溶浸泡,28.5 ℃培养箱中孵育24 h,测定尿酸含量,对具有良好XOD抑制活性的食药材做进一步降尿酸活性验证。体外试验结果表明,15种乙醇粗提物均具有XOD抑制活性,其中8种在400 μg/mL时抑制率达到50%以上,分别为:红景天、兔耳草、牡丹皮、败酱草、黄柏、绿萝花、决明子、雪菊。体内试验结果表明,8种处理组尿酸水平与模型组相比显著降低(P<0.05或P<0.01),均显示出降尿酸活性。

     

    Abstract: Through the combination of in vitro (the xanthine oxidase inhibitory activity) and in vivo (a zebrafish hyperuricemia model) methods, the uric acid-lowering activity of 15 edible herbs crude ethanol extracts was screened. In vitro experiment set up blank group, enzyme reaction group, inhibitor group and control group. The absorbance at 295 nm with microplate reader was measured. In vivo experiment 5 days post fertilization zebrafish larvae with normal development were randomly divided into blank group, model group (200 μmol/L PO+10 μmol/L XSS), edible herbs group (200 μmol/L PO+10 μmol/L XSS+extracts of different concentrations) and positive control group (200 μmol/L PO+10 μmol/L XSS+APL 2 mmol/L). All groups were cultivated at 28.5 ℃ for 24 h to determine the uric acid contents and further verify the uric acid-lowering activity. In vitro experiment showed that 15 ethanol extracts were inhibitory, with 8 having greater than 50% inhibitory rate at 400 μm/mL. The most active one was the ethanol extracts of the Rhodiola crenulate, which was followed closely by Lagotis brevituba, Poeania suffruticosa, Patrinia scabiosaefolia, Phellodendron chinense, Scindapsus aureus, Cassia tora and Coreopsis tinctorial. In vivo experiment showed that the uric acid level of all these eight extract groups were significantly lower than the model group (P<0.05 or P<0.01).

     

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