• EI
  • Scopus
  • 食品科学与工程领域高质量科技期刊分级目录第一方阵T1
  • DOAJ
  • EBSCO
  • 北大核心期刊
  • 中国核心学术期刊RCCSE
  • JST China
  • FSTA
  • 中国精品科技期刊
  • 中国农业核心期刊
  • CA
  • WJCI
  • 中国科技核心期刊CSTPCD
  • 中国生物医学SinoMed
中国精品科技期刊2020
饶川艳,于晓涵,梅丽华,等. 长寿人群饮食中膳食纤维对C57BL/6小鼠的抗氧化效果[J]. 食品工业科技,2021,42(19):342−349. doi: 10.13386/j.issn1002-0306.2020080011.
引用本文: 饶川艳,于晓涵,梅丽华,等. 长寿人群饮食中膳食纤维对C57BL/6小鼠的抗氧化效果[J]. 食品工业科技,2021,42(19):342−349. doi: 10.13386/j.issn1002-0306.2020080011.
RAO Chuanyan, YU Xiaohan, MEI Lihua, et al. Antioxidant Effect of Dietary Fiber in the Diet of Longevity Population on C57BL/6 Mice[J]. Science and Technology of Food Industry, 2021, 42(19): 342−349. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080011.
Citation: RAO Chuanyan, YU Xiaohan, MEI Lihua, et al. Antioxidant Effect of Dietary Fiber in the Diet of Longevity Population on C57BL/6 Mice[J]. Science and Technology of Food Industry, 2021, 42(19): 342−349. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020080011.

长寿人群饮食中膳食纤维对C57BL/6小鼠的抗氧化效果

Antioxidant Effect of Dietary Fiber in the Diet of Longevity Population on C57BL/6 Mice

  • 摘要: 采用6周龄健康C57BL/6小鼠研究广西巴马长寿人群膳食中膳食纤维的抗氧化效果。选取香蕉、脐橙、南瓜苗、红薯叶、空心菜、苦荬菜和茶树菇7种具有代表性的广西巴马长寿人群食物制备膳食纤维,设计了膳食纤维单一组和复合组共10种喂食方式,并使用这10种配方喂养小鼠,连续4周。试验结束后对试验小鼠血清及肝脏组织中脂过氧化产物丙二醛(malondialdehyde,MDA)、脂褐素(lipofuscin,LIP)含量,超氧化物歧化酶(superoxide dismutase, SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-Px)活力以及总抗氧化力(Total antioxidant capacity, T-AOC)进行测定。通过对比试验前后体重发现,除苦荬菜膳食纤维外,单一膳食纤维的添加组均对小鼠的体重增长无显著影响,在一定范围内,复合膳食纤维组添加量越大越能减缓小鼠体重的增长速率;其中7.5%和15%复合膳食纤维添加组小鼠心脏系数分别提高了7.22%(P<0.05)和6.92%(P​​​​​​​​​​​​​​<0.05),南瓜苗膳食纤维分离物和30%复合膳食纤维分离物组分别将小鼠脾脏系数提高了10.00%和15.00%。各组膳食纤维分离物添加组可以有效降低小鼠血清和肝脏中MDA和脂褐素Lip的水平,同时提高小鼠血清和肝脏中SOD、GSH-Px活力以及T-AOC水平。综合分析,在小鼠饮食中添加复合膳食纤维,其抗氧化应激作用更强,抗氧化效果更好。

     

    Abstract: In order to study the antioxidant effect of dietary fiber in long-lived people in Bemba, Guangxi, six-week-old healthy C57BL/6 mice were selected as study subjects. Seven kinds of dietary fiber (DF), which the most representative of longevity population in Bama, Guangxi, were selected, including banana, navel orange, pumpkin seedling, sweet potato leaf, water spinach, Ixeris sonchifolia and agrocybe chaxingu, and 10 feeding patterns were designed. Mice were fed with these 10 patterns for 4 weeks. The contents of malondialdehyde (MDA) and lipofuscin (Lip), activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in serum and liver tissues of the tested mice were determined. The results showed that except for Ixeris sonchifolia dietary fiber, the addition of single dietary fiber had no significant effect on the weight gain of mice. In a certain range, the larger the addition of compound dietary fiber, the slower the weight gain rate of mice. Among them, the heart coefficients of mice in the 7.5% and 15% compound dietary fiber addition groups were increased by 7.22 % (P<0.05) and 6.92 % (P<0.05), respectively. The spleen coefficients of mice were increased by 10.00%, 15.00% by adding pumpkin seedling dietary fiber isolate and 30% composite dietary fiber isolate. The dietary fiber isolates in each group could effectively reduce the levels of MDA and lipofuscin Lip in serum and liver of mice , and improve the activities of SOD, GSH-Px and T-AOC in serum and liver of mice. Comprehensive analysis shows that adding compound dietary fiber to the diet of mice has stronger antioxidant stress and better antioxidant effect.

     

/

返回文章
返回