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中国精品科技期刊2020
舒聪涵,相兴伟,孙继鹏,等. 金枪鱼骨胶原肽对葡聚糖硫酸钠诱导急性结肠炎小鼠的预防效果[J]. 食品工业科技,2021,42(8):326−332. doi: 10.13386/j.issn1002-0306.2020060326.
引用本文: 舒聪涵,相兴伟,孙继鹏,等. 金枪鱼骨胶原肽对葡聚糖硫酸钠诱导急性结肠炎小鼠的预防效果[J]. 食品工业科技,2021,42(8):326−332. doi: 10.13386/j.issn1002-0306.2020060326.
SHU Conghan, XIANG Xingwei, SUN Jipeng, et al. Preventive Effect of Tuna Bone Collagen Peptides on Dextran Sodium Sulfate Induced Acute Colitis in Mice[J]. Science and Technology of Food Industry, 2021, 42(8): 326−332. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060326.
Citation: SHU Conghan, XIANG Xingwei, SUN Jipeng, et al. Preventive Effect of Tuna Bone Collagen Peptides on Dextran Sodium Sulfate Induced Acute Colitis in Mice[J]. Science and Technology of Food Industry, 2021, 42(8): 326−332. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020060326.

金枪鱼骨胶原肽对葡聚糖硫酸钠诱导急性结肠炎小鼠的预防效果

Preventive Effect of Tuna Bone Collagen Peptides on Dextran Sodium Sulfate Induced Acute Colitis in Mice

  • 摘要: 目的:研究金枪鱼骨胶原肽对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导急性溃疡性结肠炎小鼠的预防效果。方法:将小鼠随机分为正常组、造模组、骨胶原肽组,正常组和造模组小鼠每日灌胃生理盐水,骨胶原肽组每日灌胃300 mg/kg的骨胶原肽。除正常组外,造模组和骨胶原肽组采用3.5%的DSS自由饮用8 d建立小鼠急性溃疡性结肠炎模型。记录每日小鼠体重,评价疾病活动指数(disease activity index,DAI),测定脏器指数,留取结肠组织测量长度并对其进行HE染色,观察病理变化。运用酶联免疫法(ELISA)检测血清和结肠组织中肠道黏膜屏障损伤指标内毒素(LPS)、二胺氧化酶(DAO)的含量以及抗氧化损伤指标超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力。结果:与正常组相比,造模组小鼠的体重下降,DAI评分明显升高,肝脾肿大,胸腺萎缩,同时结肠长度显著缩短并且病理损害严重。与造模组相比,骨胶原肽灌胃后小鼠血清和结肠组织中LPS和DAO水平均极显著降低(P<0.01),SOD和GSH-Px活力均极显著升高(P<0.01)。结论:金枪鱼骨胶原肽具有减轻急性溃疡性结肠炎症状的作用,推测其通过修复肠道损伤,提高机体抗氧化能力发挥结肠炎预防保护作用。

     

    Abstract: Objective: To study the preventive effect of tuna bone collagen peptides (TBCP) on dextran sulfate sodium (DSS) - induced acute ulcerative colitis in mice. Methods: The mice were randomly divided into normal group, model group and TBCP group. The mice in the normal group and model group were administered with normal saline every day, while the mice in the TBCP group were given 300 mg/kg bone collagen peptides daily. Except the normal group, the other two groups were treated with 3.5% DSS for 8 days to establish the acute ulcerative colitis model. During the whole experiment, the body weight of mice was recorded every day to evaluate the disease activity index (DAI). At the end of the experiment, the organs were collected to measure the organ indexs, and the colons was collected to measure the length. Also, colon paraffin slices were prepared and their pathological changes were observed under microscope after HE staining. Enzyme linked immunosorbent assay (ELISA) was used to detect the contents of LPS, DAO and the activities of SOD and GSH-Px in serum and colon tissue, respectively. Results: Compared with the normal group, the weight of mice in the model group decreased and DAI scores were significantly increased. The livers and spleens of mice in the model group were swollen and the thymus gland was atrophic. Meanwhile, the colon length were significantly shortened and pathological damage was serious. Compared with the model group, the levels of LPS and DAO in serum and colon tissue of mice in TBCP group were significantly decreased (P<0.01), and the activities of SOD and GSH-Px were significantly increased (P<0.01). Conclusion: The tuna bone collagen peptides could reduce the symptoms of acute ulcerative colitis, and it is speculated that tuna bone collagen peptides could play a role in the prevention of colitis by repairing intestinal injury and improve antioxidant capacity.

     

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